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KMID : 0900919990230030229
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Korean journal of Animal Reproduction
1999 Volume.23 No. 3 p.229 ~ p.237
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Effects of Ascorbic Acid and Ferrous Sulfate on In-Vitro Fertility of Frozen-Thawed Spermatozoa in Porcine
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Nam H.-S.
Park C.-K.
Cheong H.-T.
Lee S.-C.
Kim J.-H.
Yang B.-K.
Kim C.-I.
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Abstract
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The influence of ascorbic acid (Asc) and ferrous sulfate (Fe^{2＋}) on capacitation, acrosome reaction and fertility in vitro was investigated in boar frozen-thawed spermatozoa with or without preincubation. The addition of 0-1.0 mM Fe^{2＋} to sperm suspensions during preincubation increase acrosome reaction (P＜0.05) and oocyte penetration. These increase are also associated with addition of 0~0.5 mM Asc, but the penetration rates were higher in those without than with sperm preincubation. The addition of 0.1 mM Asc than 0.5 mM in medium with Fe^{2＋} were significantly (P＜0.05) higher on acrosome reaction at 2 h after sperm preincubation. No significant differences, however, were observed in penetration rates among the concentrations of Asc. On the other hand, when preincubation medium containing the Asc was supplemented with 0.1mM Fe^{2＋}, the percentage of spermatozoa acrosome-reacted were significantly (P＜0.05) higher than in medium wothout Fe^{2＋}, on the contrary, the penetration rate was significantly (P＜0.05) low during in-vitro fertilization. These findings indicate some apparent effects of Fe^{2＋} or Asc addition on acrosome reaction and the fertilizing potential by sperm preincubation.
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KEYWORD
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Ascorbic acid, Acrosome reaction, Capacitation, Ferrous sulfate, Porcine
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